| Prefect: |   Cardinal João Bráz de Aviz (78) (2011.01.04 – 2025.01.06) |
| Prefect: |  Cardinal Franc Rode, C.M. (91) (2004.02.11 – 2011.01.04) |
| Prefect: |  Cardinal Eduardo Martínez Somalo (1992.01.21 – 2004.02.11) |
| Prefect: |  Cardinal Jean Jérôme Hamer, O.P. (1985.05.27 – 1992.01.21) |
| Prefect: |  Blessed Cardinal Eduardo Francisco Pironio (1976.05.29 – 1984.04.08) |
| Prefect: | Cardinal Arturo Tabera Araoz, C.M.F. (1973.09.08 – 1975.06.13) |
| Prefect: |  Cardinal Ildebrando Antoniutti (1963.07.26 – 1973.09.08) |
| Prefect: | Cardinal Valerio Valeri (1953.01.17 – 1963.07.22) |
| Prefect: | Cardinal Clemente Micara (1950.11.11 – 1953.01.17) |
| Prefect: | Cardinal Luigi Lavitrano (1945.05.14 – 1950.08.02) |
| Prefect: | Cardinal Vincenzo La Puma (1935.12.31 – 1943.11.04) |
| Prefect: |  Cardinal Alexis-Henri-Marie Lépicier, O.S.M. (1928.12.17 – 1935.12.31) |
| Prefect: | Cardinal Camillo Laurenti (1922.07.05 – 1928.12.17) |
| Prefect: | Cardinal Teodoro Valfrè di Bonzo (1920.03.06 – 1922.06.25) |
| Prefect: | Cardinal Raffaele Scapinelli di Leguigno (1918.12.19 – 1920.03.06) |
| Prefect: |  Cardinal Giulio Tonti (1917.02.13 – 1918.12.11) |
| Prefect: | Cardinal Diomede Falconio, O.F.M. (1916.02.26 – 1917.02.08) |
| Prefect: | Cardinal Domenico Serafini, O.S.B. Subl. (1916.01.27 – 1916.02.26) |
| Prefect: | Cardinal Ottavio Cagiano de Azevedo (1913.10.31 – 1915.12.06) |
| Prefect: | Cardinal José de Calasanz Félix Santiago Vives y Tutó, O.F.M. Cap. (1908.10.26 – 1913.09.07) |
| Pro-Prefect: | Cardinal Jean Jérôme Hamer, O.P. (1984.04.08 – 1985.05.27) |
| Pro-Prefect: | Blessed Cardinal Eduardo Francisco Pironio (1975.09.20 – 1976.05.29) |
| Pro-Prefect: | Cardinal Vincenzo La Puma (1935.12.22 – 1935.12.31) |
| Pro-Prefect: | Cardinal Ottavio Cagiano de Azevedo (1913.06.12 – 1913.10.31) |
| Secretary: | Sr. Simona Brambilla, I.S.M.C. (61) (2023.10.07 – 2025.01.06) |
| Secretary: |  Archbishop José Rodríguez Carballo, O.F.M. (72) (2013.04.06 – 2023.10.07) |
| Secretary: |   Archbishop Joseph William Tobin, C.SS.R. (73) (later Cardinal) (2010.08.02 – 2012.10.18) |
| Secretary: | Archbishop Gianfranco Agostino Gardin, O.F.M. Conv. (2006.07.10 – 2009.12.18) |
| Secretary: | Archbishop Piergiorgio Silvano Nesti, C.P. (1996.11.27 – 2006.07.10) |
| Secretary: |  Archbishop Francisco Javier Errázuriz Ossa, I.Sch. (92) (later Cardinal) (1990.12.22 – 1996.09.24) |
| Secretary: | Archbishop Vincenzo Fagiolo (later Cardinal) (1984.04.08 – 1990.12.15) |
| Secretary: |  Bishop Paul Augustin Mayer, O.S.B. (later Cardinal) (1971.09.08 – 1984.04.08) |
| Secretary: | Fr. Edward Louis Heston, C.S.C. (later Archbishop) (1969 – 1971.09.08) |
| Secretary: | Archbishop Antonio Mauro (1967.07.04 – 1969.04.12) |
| Secretary: | Archbishop Paul-Pierre Philippe, O.P. (later Cardinal) (1959.12.14 – 1967.06.29) |
| Secretary: | Fr. Arcadio María Larraona, C.M.F. (later Cardinal) (1950.12.11 – 1959.12.14) |
| Secretary: | Archbishop Luca Ermenegildo Pasetto, O.F.M. Cap. (later Patriarch) (1935 – 1950.11.11) |
| Secretary: | Msgr. Vincenzo La Puma (later Cardinal) (1925.04.07 – 1935.12.16) |
| Secretary: | Abbot Mauro Serafini, O.S.B. Subl. (1918 – 1925) |
| Secretary: | Bishop Adolfo Turchi (later Archbishop) (1916 – 1918.07.17) |
| Secretary: |  Archbishop Donato Sbarretti (later Cardinal) (1910.10.29 – 1916.12.04) |
| Undersecretary: | Fr. Pier Luigi Nava, S.M.M. (2018.11.27 – 2023.11) |
| Undersecretary: | Sr. Nicoletta Vittoria Spezzati, A.S.C. (2011.12.17 – 2018.02.23) |
| Undersecretary: | Fr. Sebastiano Paciolla, O. Cist. (2008.02.28 – 2018.10.22) |
| Undersecretary: |  Fr. Vincenzo Bertolone, S.d.P. (79) (later Archbishop) (2004.06.12 – 2007.03.10) |
| Undersecretary: | Sr. Enrica Rosanna, F.M.A. (2004.04.24 – 2011.10.15) |
| Undersecretary: |  Msgr. Josef Clemens (78) (later Bishop) (2003.02.12 – 2003.11.25) |
| Undersecretary: | Msgr. Juan José Dorronsoro Allo (1988 – 2003) |
| Undersecretary: | Msgr. Joseph Anthony Galante (later Bishop) (1986 – 1992.10.13) |
| Undersecretary: | Fr. Jesús Torres Llorente, C.M.F. (1984 – 2004.04.24) |
| Undersecretary: | Msgr. Mario Albertini (1974 – 1988) |
| Undersecretary: | Fr. Basil Mary Heiser, O.F.M. Conv. (1972 – 1984) |
| Undersecretary: |  Msgr. Dorio-Marie Huot, S.M.M. (1967 – 1971) |
| Undersecretary: | Msgr. Giovanni Battista Verdelli (1958 – 1971) |
| Undersecretary: | Msgr. Pietro Palazzini (later Cardinal) (1956.12.22 – 1958.12.18) |
| Undersecretary: | Msgr. Giovanni Battista Scapinelli di Léguigno (later Archbishop) (1950 – 1956) |
| Undersecretary: | Fr. Arcadio María Larraona, C.M.F. (later Cardinal) (1943.11.27 – 1950.12.11) |
| Undersecretary: | Msgr. Vincenzo Padovani (1935? – 1943) |
| Undersecretary: | Msgr. Enrico Caiazzo (1925 – 1935?) |
| Undersecretary: | Msgr. Vincenzo La Puma (later Cardinal) (1915 – 1925.04.07) |
| Undersecretary: | Msgr. Francesco Cherubini (later Archbishop) (1910? – 1915.12.09) |
| Undersecretary: | Msgr. Oreste Giorgi (later Cardinal) (1907.06.03 – 1911.12.07) |
| Adjunct Undersecretary: | Fr. Elio Gambari, S.M.M. (1972 – 1983) |
| Bureau Chief: |  Msgr. Orazio Pepe (61) (2009.11.05 – ?) |
| Bureau Chief: |  Fr. Waldemar Barszcz, T.O.R. (2007.11.10 – ?) |
| Bureau Chief: |  Fr. Eusebio Ignacio Hernández Sola, O.A.R. (81) (later Bishop) (1995 – 2011.01.29) |
(x 40)
